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asnO [2019-08-26 13:10:39]
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asnO [2019-08-26 13:10:39]

asparagine synthase (glutamine-hydrolysing)
locus
BSU_10790
pI
5.70
mw
70.54 kDa
protein length
614 aa Sequence Blast
gene length
1845 bp Sequence Blast
function
biosynthesis of asparagine
product
asparagine synthase (glutamine-hydrolysing)
essential
no
ec
6.3.5.4
synonyms
yisO,yucB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.5|Biosynthesis/ acquisition of aspartate/ asparagine]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    1,157,237 1,159,081

    Phenotypes of a mutant

  • inactivation of ''[gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]'' renders cells asporogenous [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)

    • Protein family

  • asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|AsnB] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|AsnH], according to UniProt)

    • Structure

  • [PDB|1CT9] (from E. coli, 27% identity) [pubmed|10587437]

    • Expression and Regulation

    Operons

    genes
    [gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]
    description
    [Pubmed|10498721]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|10498721], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|10498721]
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    Biological materials

    Mutant

  • BKE10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA
  • BKK10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA

    • References

    Reviews

  • 12859215,11395405

    • Original publications

  • 10498721,15383836,26735940,10587437